PFT401 - Microbiology I

Prerequisite(s)
None

Topic Outline
7 WEEK DELIVERY MODE
Test and assignment dates; Introduction to microbiology; bacteria, viruses, yeasts and moulds, procaryotes vs. eucaryotes, classification systems, theory of spontaneous generation
Safety rules for the laboratory, laboratory conditions for working with microorganisms, levels of containment, WHMIS overview. Bacterial morphology: Introduction to the microscope, size and staining characteristics, growth and nutritional requirements, liquid vs. solid media. Cultivation of bacteria: major characteristics of bacteria, classification based on nutritional and physical conditions requirements, differential and selective media
Microscope, Exp. #1, examination of prepared slides, parfocality, resolution, handling and microscope operation. Preparation of media, Exp. #2
.
Principles of microscopy, parts of a microscope, magnification, resolution and index of refraction, examples of modern light microscopes, fluorescent, phase and electron microscopes; bacterial cell components: internal structures, cell membranes and cell walls, Gram-negative vs. Gram-positive bacteria, bacterial spores, flagella, pili, capsules and sheaths, pure cultures
Subculture to broths and slopes, Exp. #3.
Selective and differential media, Exp. #4.
Streak plates, Exp. #5.
Simple and Gram-stain, Exp. #6.

Cell division and growth cycle of microorganisms: bacterial growth, stationary, synchronous and continuous cultures, replication of viruses, yeasts and moulds, calculations of generations, generation times and growth rate of bacteria;  basis for bacterial identification: bacterial enzymes, morphology, biochemistry and physiology. Spore stain, Exp. #7.
Antimicrobial action of chemical substances and physical conditions, Exp. #8.

Bergy's Manual and its use, major groups of bacteria, review

Quantitative measurement of bacterial growth: direct microscope counts, electronic counts, plate counts and turbidimetric methods;  fundamentals and methods of bacterial growth control: physical and chemical methods, examples of applications in the pharmaceutical industry; estimation of bacterial growth by dilution plate count and turbidimetry, Exp. #9.

Antibiotics and chemotherapeutic agents: types of antimicrobial agents, mode of action and mechanism of resistance, side effects and problems associated with resistance; bacterial genetics: transformation, transduction and recombination, mutation, DNA homology tests; bacteria most often associated to human diseases: examples of Gram-positive, Gram-negative, acid-fast bacillus
Antibiotics sensitivity testing and evaluation of antiseptics and disinfectants, Exp. #10.

Review of laboratory results

14 WEEK DELIVERY MODE
Introduction to microbiology, test and assignment dates, bacteria, viruses, yeasts and moulds, procaryotes vs. eucaryotes, classification systems, theory of spontaneous generation.
Safety rules for the laboratory, WHMIS overview, the microscope
Microscopic Exp. #1, examination of prepared slides
Parfocality, resolution, how to handle and operate the microscope

Bacteria and how to recognize them: morphology, size and staining characteristics of bacteria, growth and nutritional requirements. Fluorescent, phase, and electron microscopes
Liquid vs. solid media preparation of media, Exp. #2.

Cultivation of bacteria,  nutritional types of bacteria; physical conditions required for growth; differential and selective media, major characteristics of bacteria subculture to broths and slopes, Exp. #3.

Bacterial cell components internal structure, cell membranes and cell walls, gram negative vs. gram positive bacteria, bacterial spores, flagella, pili, capsules and sheaths pure cultures
streak plates, Exp. #4.

Growth of micro-organisms, cell division and growth cycles of bacteria, stationary, synchronous and continuous cultures replication of viruses, yeasts and moulds gram-stains, Exp. #5 spore stain, Exp. #6.

Calculations of generations, generation times and growth rate of bacteria bacterial enzymes, anaerobes and aerobes free oxygen and bacterial growth, Exp. #7.

Bergy's Manual and its use major groups of bacteria,  review

Quantitative measurement of bacterial growth direct microscope counts, electronic counts plate counts and turbidimetric methods dilutions and suspensions of bacteria, Exp. #8.

Fundamentals and methods of control of bacteria physical and chemical means demonstration of bacterial enzymes, Exp. #9a bacteriostatic dyes, Exp. #9b.

Antibiotics and chemotherapeutic agents types of antimicrobial agents, mode of action of important agents. Reactions, side effects and problems associated with resistance
antibiotics, Exp. #10.

Bacterial genetics transformation, transduction and recombination
mutations, DNA homology tests

Classification of bacteria ordinary Gram-positive and Gram-negative bacteria

Review

Mode of Instruction

• Lectures to teach the basics in microbiology
• Introduction to the fundamental principles behind each experiment
• Students work in pairs to conduct each assigned experiment
• Hand-outs of lab exercise protocols
• Quizzes on experiments are used to determine students' understanding of the lab work

Prescribed Texts
(Pkg) MICROBIOLOGY AN INTRODUCTION W/MASTERING MICROBIOLOGY,  11th Edition; Tortora/Funke/Case; Publisher: Addison Wesley Longman   ISBN 978-0-321-76738-7 Pearson Education Canada

To find out the cost of books and learning material go here.

Any courses not listed on the bookstore webpage do not require any resources for purchase. All resources will be provided by your instructor.

Reference Material
None

Required Supplies
All students must wear a laboratory coat and safety glasses while in the lab.  This requirement is mandatory and consistent with safe practice.  Anyone not having the mandatory requirements will be asked to leave the lab.

Student Progression and Promotion Policy

http://www.senecapolytechnic.ca/about/policies/student-progression-and-promotion-policy.html

Grading Policyhttp://www.senecapolytechnic.ca/about/policies/grading-policy.html

A+	90%  to  100%
A	80%  to  89%
B+	75%  to  79%
B	70%  to  74%
C+	65%  to  69%
C	60%  to  64%
D+	55%  to  59%
D	50%  to  54%
F	0%    to  49% (Not a Pass)
OR
EXC	Excellent
SAT	Satisfactory
UNSAT	Unsatisfactory

For further information, see a copy of the Academic Policy, available online (http://www.senecapolytechnic.ca/about/policies/academics-and-student-services.html) or at Seneca's Registrar's Offices. (https://www.senecapolytechnic.ca/registrar.html).

Modes of Evaluation
Assignments

• Assignments are due at the beginning of the class on which they are due.
• A late penalty of 10% per day is assessed for late assignments, including those not handed in at the beginning of class when due.
• Material will not be accepted after one week following the due date and/or when the marked material is returned to students, whichever comes first.
• Assignments are to be prepared by computer.

Absenteeism and Exams

• Students should be aware that absenteeism almost guarantees an inability to achieve satisfactory grades.
• Students who are absent for an examination due to an emergency (e.g., motor vehicle accident, hospitalization or death in the family) may provide official documentation within five days of the missed exam and be provided a deferred exam at a later date.  Official documentation includes a death notice or an original doctor’s certificate identifying the date, length of time expected absence and the specific reason for the absence.  Examinations missed without official documentation and approval result in a grade of zero.
• There are no deferred options for missed tests.  The value of missed tests, at the discretion of the Faculty, will be added to other evaluation components

English Proficiency

• All written work should demonstrate the following characteristics for clarity and conciseness:

Format for Assignments

• Students must use the standard, APA style for quoting sources.   Help is available at:  http://library.senecapolytechnic.ca

LAB COURSES
Laboratory Attendance
The laboratory component is essential and therefore it is strongly recommended  that you attend all labs.  Any missed labs must be supported with a legal document with three days of the lab.  Any student who fails to attend 2 scheduled laboratory classes for a 7 week subject and more than 3 laboratory classes for a 14 week subject will not pass the subject.    

Laboratory Safety
Students are required to review and understand the safety procedures and guidelines outlined on the first class and sign the sheet to this effect before beginning work in the laboratory.  Students must also wear a lab coat and safety glasses when conducting experiments.
A student who arrives without the proper safety equipment will not be permitted to participant in the lab but will be asked to leave the class.  The student will receive no grade for the lab missed.

Grading is based on the following marking scheme:

Quizzes (total 4 quizzes, average of the highest 3)	20%
Mid-Term Test	30%
Laboratory Skills	10%
Final Exam	40%

NOTE:  DATES FOR TESTS AND QUIZZES WILL BE PROVIDED BY THE INSTRUCTOR

Other Evaluation Considerations
The student is expected to comply with the Safety Rules for working in the laboratory, sign the safety contract, and WILL NOT UNDER ANY CIRCUMSTANCES WEAR CONTACT LENSES in the laboratory.  The student will know where all safety equipment is located in the laboratory and will be familiar with WHMIS concepts and signage.

PLEASE RETAIN THIS SUBJECT OUTLINE FOR POSSIBLE FUTURE USE IN SUPPORT OF CREDIT APPLICATIONS AT OTHER EDUCATIONAL INSTITUTIONS